Protocol

Using Phenol and Ether to Concentrate Proteins and Remove Interfering Substances

This protocol was adapted from "Using Phenol and Ether to Concentrate Proteins and Remove Interfering Substances," contributed by Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia, Appendix 2, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

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INTRODUCTION

This method (Sauvé et al. 1995) is effective for recovering proteins from dilute solutions containing 10 ng/ml of protein (in) 0.5 Triton X-100, 0.5% SDS, or 1 M NaCl.

MATERIALS

Reagents

Ether (AR grade)

Neutral or alkaline buffer

Please see Step 10.

Test protein samples

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Equipment

METHOD