Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4258

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Using Deoxycholate and Trichloroacetic Acid to Concentrate Proteins and Remove Interfering Substances

Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research

This protocol was adapted from "Using DOC and TCA to Concentrate Proteins and Remove Interfering Substances," contributed by Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia, Appendix 2, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol is a modification (Peterson 1983) of the procedure of Bensadoun and Weinstein (1976). The DOC/TCA precipitation method allows the quantitative recovery of proteins below 1 µg/ml.


MATERIALS

Reagents

Neutral or Alkaline . . . [Full Text of this Article]

Equipment


METHOD


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