Protocol

Using Trichloroacetic Acid to Concentrate Proteins and Remove Interfering Substances

This protocol was adapted from "Using TCA to Concentrate Proteins and Remove Interfering Substances," contributed by Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia, Appendix 2, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

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INTRODUCTION

The two most commonly used methods for concentrating dilute protein solutions for quantitative and qualitative analyses involve precipitation in the presence of acids or organic solvents, resulting in protein denaturation. The reduced solubility of denatured proteins allows their recovery in a . . . [Full Text of this Article]

MATERIALS

Reagents

Equipment

METHOD