Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.ip15

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Measuring FRET by Acceptor Photobleaching

Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens

This information panel was adapted from "Imaging Protein Interactions by FRET Microscopy," Chapter 32, in Protein-Protein Interactions (ed. Golemis and Adams). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.

Occurrence of FRET leads to a decrease in the intensity of the donor fluorescence that is proportional to the number of donor-tagged molecules that interact with an acceptor-bound molecule. Experimentally, the decrease in intensity can be measured on the specimen by specifically photobleaching the acceptor (by excitation at its absorption maximum) and acquiring . . . [Full Text of this Article]


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Related Article

Measuring FRET by Sensitized Emission
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
CSH Protocols 2006: 14. [Extract] [Full Text]