Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4465

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Flow Cytometric Analysis of Mitochondrial Transmembrane Potential ({Delta}{Psi}m)

Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel and Douglas R. Green

This protocol was adapted from "Apoptosis Assays," Chapter 15, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998. This three-volume set is now out of print; however, some of the microscopy methods were republished in Basic Methods in Microscopy, by David L. Spector and Robert D. Goldman.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for evaluation of mitochondrial function using the fluorochrome CMXRos. CMXRos is sequestered by actively respiring mitochondria, but washed out when the mitochondrial membrane potential is lost. This analysis can be combined with the TUNEL technique or immunocytochemistry. (Please see TUNEL Staining of Adherent Cells to . . . [Full Text of this Article]


MATERIALS

Reagents


METHOD


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