Protocol

Growth and Concentration of Chlorogonium for Culturing Freshwater Hypotrichs

This protocol was adapted from "Culture and Manipulation of Hypotrichous Ciliated Protozoa," Chapter 17, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998. This three-volume set is now out of print; however, some of the microscopy methods were republished in Basic Methods in Microscopy, by David L. Spector and Robert D. Goldman.

The first 15% of the full text of this article appears below.

INTRODUCTION

This protocol describes the growth and concentration of the alga Chlorogonium elongatum as a food source for culturing freshwater hypotrichs. Most freshwater hypotrichs (including Oxytricha nova, O. fallax, and O. trifallax; Euplotes aediculatus and E. eurystomous; and Stylonychia lemnae) can be grown to high density with Chlorogonium as the food organism. A similar regimen can be used to prepare other food sources such as Tetrahymena or bacteria (e.g., Aerobacter aerogenes).

MATERIALS

Reagents

Algae medium

. . . [Full Text of this Article]

Equipment

METHOD