Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4427

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Disaggregating Cleavage-Stage Embryos and the Inner Cell Mass (ICM) of Blastocysts into Individual Cells

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Production of Chimeras," Chapter 11, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for disaggregating embryos and the ICM of blastocysts into individual cells.


MATERIALS

Reagents

Bovine serum albumin (BSA)

recipe Calcium-free M16 medium

recipe M16 medium

Mouse embryos that have been allowed to recover for ~1 hour after removing the zona pellucida . . . [Full Text of this Article]

Equipment


METHOD


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Removal of Zona Pellucida
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4421. [Extract] [Full Text]

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Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4428. [Extract] [Full Text]