Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4425

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Assembling Aggregates between Diploid and Tetraploid Embryos

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Production of Chimeras," Chapter 11, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for producing diploid embryo-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for diploid embryo-diploid embryo aggregation. The resulting chimeras are useful for phenotypic analysis when an induced mutation has an extraembryonic phenotype.


MATERIALS

Reagents

Embryos, diploid, 2.5-days post coitum (dpc) (see Collecting Two-Cell- to Compacted Morula-Stage Embryos)

Embryos, tetraploid (see Production of . . . [Full Text of this Article]

Equipment


METHOD


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