Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4417

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Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Production of Chimeras," Chapter 11, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

Injection needles require sharp tips that allow the blastocyst to be penetrated easily. This protocol describes a method of creating sharp injection needle tips.


MATERIALS

Equipment

Pulled injection needles (see Making Injection Pipettes)

Scalpel, disposable

Silicon tubing, very thick (external diameter 15-20 mm)

Stereo . . . [Full Text of this Article]


METHOD


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Related Protocol

Making Injection Pipettes
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4395. [Extract] [Full Text]