Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4416

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Preparation of Embryonic Stem (ES) Cells for Injection

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Production of Chimeras," Chapter 11, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

Injection of ES cells into the cavity of blastocysts is one of the main methods for generating chimeras. This protocol describes the preparation of ES cells for injection.


MATERIALS

Reagents

ES cells, subconfluent, grown on 6-cm tissue culture dish with mouse embryo fibroblast (MEF) feeder layer (see De Novo Isolation of Embryonic Stem (ES) Cell Lines from Blastocysts. . . [Full Text of this Article]

Equipment


METHOD


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Related Protocol

De Novo Isolation of Embryonic Stem (ES) Cell Lines from Blastocysts
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4403. [Extract] [Full Text]