Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4298

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Immunoblotting: Preparing Protein Solutions

Ed Harlow and David Lane

This protocol was adapted from "Immunoblotting," Chapter 8, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

Most sources of antigens can be mixed with a gel electrophoresis sample buffer and analyzed directly using immunoblots. In the approach described here, proteins are mixed with a sample buffer and prepared for electrophoresis on standard Tris/glycine SDS-polyacrylamide gels (see SDS-Polyacrylamide Gel Electrophoresis . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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