Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4290

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Growing Adherent Cells Directly on Tissue Dishes

Ed Harlow and David Lane

This protocol was adapted from "Staining Cells," Chapter 5, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

For low-resolution work, cells to be used for staining can be grown directly on regular tissue-culture dishes. It is a convenient method that does not require much preparatory work. Because of the poorer optical . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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