Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4289

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Growing Adherent Cells on Coverslips or Multiwell Slides

Ed Harlow and David Lane

This protocol was adapted from "Staining Cells," Chapter 5, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

Glass is an excellent substrate for most tissue-culture-adapted cells and is compatible with all fixing and staining solutions. Glass coverslips in tissue-culture dishes or in 24-well multiwell plates are suitable carriers, as are multiwell slides. For high-resolution studies, choose glass coverslips of the highest available grade; #1 or #1.5 coverslips are the appropriate thickness for most . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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