Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4074

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Related Collections
Right arrow Molecular Biology, general
Right arrow Bacteriophage lambda
Right arrow cDNA
Right arrow Libraries
Right arrow Libraries, general
Right arrow cDNA Libraries
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Construction of cDNA Libraries

Stage 6: Ligation of cDNA to Bacteriophage {lambda} Arms

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

The final stage of cDNA library construction involves optimizing ligation of the size-fractionated cDNA to bacteriophage {lambda} arms, followed by packaging and plating of the recombinant bacteriophages.


MATERIALS

E. coli strain, freshly prepared overnight cultures

Use strains C600 (BNN93) for growth and BNN102 (C600hflA) for screening of cDNA libraries constructed in bacteriophage {lambda}gt10. Use strain Y1090hsdR for growth and screening of cDNA libraries constructed in bacteriophage {lambda}gt11. Use strain BB4 for growth and screening of cDNA libraries constructed in {lambda}ZAP and {lambda}ZAPII or strain XL1-Blue . . . [Full Text of this Article]


METHOD


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?