Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4064

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Purification of Radiolabeled Oligonucleotides by Chromatography on a Sep-Pak C18 Column

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Radiolabeled oligonucleotides can be separated from unincorporated radiolabel by chromatography on silica gel resins. The protocol is suitable only for purifying oligonucleotides carrying 5'-phosphate groups. The method described in Purification of Synthetic Oligonucleotides by Polyacrylamide Gel Electrophoresisshould be used to purify oligonucleotides with free 5'-hydroxyl groups.


MATERIALS

caution Acetonitrile (5%, 30%, and 100%)

. . . [Full Text of this Article]


METHOD


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