Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4038

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Preparation of Genomic DNA from Mouse Tails and Other Small Samples

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

This simple protocol is used to extract DNA from small numbers of cultured cells and from fragments of soft or bony tissues. The method is used chiefly to genotype transgenic and knockout mice. Each 6-10-mm snippet of mouse tail yields 50-100 µg of DNA that can be used in dot or slot blotting to detect a transgene of interest, in Southern hybridization to detect DNA fragments that are <20 kb in size, and as a template in PCRs.


MATERIALS

Isopropanol

recipe caution Phenol:chloroform:isoamyl alcohol (25:24:1 v/v)

recipe SNET

recipe TE (pH 8.0)

Ethanol

Optional, please see Step 5.

PBS

Required for cells grown in suspension . . . [Full Text of this Article]


METHOD


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