Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4032

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Pulsed-field Gel Electrophoresis via Transverse Alternating Field Electrophoresis Gels

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

In this form of pulsed-field gel electrophoresis, electrodes are positioned on opposite sides of a vertically oriented gel. The DNA moves first toward one electrode and then toward the other, forming a zigzag pattern. The vector of this oscillation is a straight line from the loading well to the base of the gel. Variation in voltage and pulse time allows separation of DNAs ranging in size from 2 kb to >6000 kb. This protocol, supplied by Tommy Hyatt and Helen Hobbs (University of Texas Southwestern Medical Center, Dallas), describes the resolution of genomic DNA by TAFE, followed by blotting and . . . [Full Text of this Article]


MATERIALS


METHOD


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