Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3941

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Addition of Synthetic Linkers to Blunt-ended DNA

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Linkers are small self-complementary pieces of synthetic DNA, usually 8-16 nucleotides in length, that anneal to form blunt-ended, double-stranded molecules containing a restriction site. Linkers are used to equip blunt-ended termini of DNA with restriction sites as an aid to cloning.


MATERIALS

recipe ATP (5 mM and 10 mM)

Omit 5 mM from the ligation reaction in Step . . . [Full Text of this Article]


METHOD


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