Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3937

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Screening Bacterial Colonies by Hybridization: Large Numbers

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

This procedure is used to plate, replicate, and subsequently screen large numbers of bacterial colonies (up to 2 x 104 colonies per 150-mm plate or 104 colonies per 90-mm plate).


MATERIALS

E. coli strain, transformed with recombinant plasmids, as culture

(Use bacteria transformed by one of the methods described in The Hanahan Method for Preparation and Transformation of Competent E. coli: High-efficiency Transformation, The Inoue Method for Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells, Preparation and Transformation of Competent E. coli Using Calcium Chlorideand Transformation of E. coli by Electroporation) OR Amplified aliquot of . . . [Full Text of this Article]


METHOD


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