Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3920

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Attaching Adaptors to Protruding Termini

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Adaptors are short double-stranded synthetic oligonucleotides that carry an internal restriction endonuclease recognition site and single-stranded tails at one or both ends. Adaptors are used to exchange restriction sites at the termini of linear DNA molecules. They may be purchased in phosphorylated and unphosphorylated forms.


MATERIALS

recipe 10x Linker kinase buffer

recipe ATP (10 . . . [Full Text of this Article]


METHOD


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