Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3916

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Removal of Small Fragments of Nucleic Acid from Preparations of Plasmid DNA by Centrifugation through NaCl

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Contamination of plasmid DNA by fragments of DNA and RNA is reduced to an acceptable level by centrifugation through 1 M sodium chloride. This method was devised by Brian Seed when he was a graduate student at Harvard University.


MATERIALS

DNA sample, purified through CsCl gradient

Use material from either Step 14 of Removal of Ethidium Bromide from DNA by Extraction . . . [Full Text of this Article]


METHOD


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