Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3915

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Removal of Ethidium Bromide from DNA by Ion-exchange Chromatography

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Ethidium bromide is removed from DNA by chromatography through a cation-exchange resin.


MATERIALS

DNA sample, purified through CsCl gradient

Use material from either Step 7 of Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients: Continuous Gradientsor Step 5 of Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients: Discontinuous Gradients.

Ethanol

Optional, please see Step 5.

recipe caution HCl (1 N)

recipe NaCl (5 M)

Store the solution of . . . [Full Text of this Article]


METHOD


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