Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3907

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Purification of Plasmid DNA by Precipitation with Polyethylene Glycol

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Crude preparations of plasmid DNA are first treated with lithium chloride and RNase (to remove RNA). The plasmid DNA is then precipitated in a solution containing polyethylene glycol and MgCl2.


MATERIALS

caution Chloroform

Crude plasmid preparation

Use material from either Step 17 of Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparationor Step 19 of Preparation of Plasmid DNA by Large-scale Boiling Lysis.

Ethanol

Optional, please see Step 5.

Isopropanol

recipe caution LiCl (5 M)

recipe . . . [Full Text of this Article]


METHOD


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