Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3894

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Analysis of Interacting Proteins with SPR Spectroscopy Using BIAcore

Stage 1: Preparation of the Capture Surface and Test Binding

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

The introduction of commercial instruments, such as BIAcore, that are capable of measuring surface plasmon resonance (SPR), has simplified the study of macromolecular interactions by providing a format that may be used to measure molecular interactions in real time with small analytical amounts of material. This protocol describes the sequence of steps for analyzing a typical antibody-antigen system in a BIAcore experiment. The antibody (antithyroid stimulating hormone [anti-TSH]) and its ligand (TSH) are available commercially and can be used to optimize the parameters of the system. A complete description of the setup and use of the instrument may be found . . . [Full Text of this Article]


MATERIALS


METHOD


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