Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3651

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Exon Trapping and Amplification

Stage 5: Analysis of Clones

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

The final stage of this protocol involves amplification and sequencing of the exon-trapped products. This protocol was provided by Deanna M. Church (Church and Buckler 1999).


MATERIALS

recipe 10x Amplification buffer

Include 0.01% (w/v) gelatin in the buffer.

Taq DNA polymerase

Exon-trapped products cloned into a pBSII vector

Clones are in the form of recombinant DH5{alpha} colonies prepared in Exon Trapping and Amplification Stage 4: Reverse . . . [Full Text of this Article]


METHOD


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