Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3642

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Assay for Luciferase in Extracts of Mammalian Cells

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

In this protocol, cells transfected with a luciferase reporter plasmid are lysed in a detergent-containing buffer. Luciferase in the extract catalyzes an oxidation reaction in which D-luciferin is converted to oxyluciferin, with production of light at 556 nm that can be quantified in a luminometer.


MATERIALS

Cultured mammalian cells transfected with the DNA of interest

The cells should be transfected with a luciferase . . . [Full Text of this Article]


METHOD


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