Protocol

Rapid Isolation of Mammalian DNA

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

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INTRODUCTION

Mammalian DNA prepared from blood or tissues as described in this protocol is 20-50 kb in size and suitable for use as a template in PCRs. The yields of DNA vary between 0.5 and 3.0 µg/mg tissue or 5 and 15 µg per 300 µl of whole blood.

MATERIALS

DNase-free RNase (4 mg/ml)

Ethanol

Optional, please see Step 5.

Isopropanol

Mammalian tissue

Potassium acetate (5 M)

Proteinase K (20 mg/ml)

Use of a genomic grade proteinase K that has been shown to be free of DNase and RNase activity.

Rapid . . . [Full Text of this Article]

METHOD