Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3297

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Working with Yeast Artificial Chromosomes

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol outlines a procedure to validate and store yeast strains carrying YACs.


MATERIALS

S. cerevisiae carrying recombinant YAC clone

recipe CM

recipe Glycerol (30% v/v) in YPD medium

recipe YPD agar plates

recipe YPD medium

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To prepare liquid or agar medium, mix the ingredients in a final volume of 1 liter of . . . [Full Text of this Article]


METHOD


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