Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3222

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Markers for Pulsed-field Gel Electrophoresis

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Markers for pulsed-field gel electrophorsis can be generated by ligation of linear monomers of bacteriophage {lambda} DNA (48.5 kb) into a nested series of concatemers. This procedure yields a series of concatemers that contain up to 20 tandemly arranged copies of bacteriophage DNA.


MATERIALS

recipe 1x Ligation buffer with PEG

recipe . . . [Full Text of this Article]


METHOD


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