Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3214

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Related Collections
Right arrow Molecular Biology, general
Right arrow DNA Purification
Right arrow Electrophoresis, general
Right arrow Electrophoresis of Nucleic Acids
Right arrow Electrophoresis of Nucleic Acids, general
Right arrow Electrophoresis of DNA
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Recovery of DNA from Agarose Gels: Electrophoresis onto DEAE-cellulose Membranes

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

Recovery of bands of DNA from agarose gels by electrophoresis onto a sliver of DEAE-cellulose membrane can be performed simultaneously on many samples and reliably gives high yields of fragments between 500 bp and 5 kb in length.


MATERIALS

recipe 6x Gel-loading buffer

recipe caution Ammonium acetate (10 M)

Optional, please see Step 3.

recipe Carrier RNA

recipe DEAE high-salt elution buffer

recipe DEAE low-salt wash buffer

DNA sample

DNA standards

recipe EDTA (10 mM, pH 8.0)

Ethanol

Optional, please see Step 5.

recipe caution NaOH (0.5 N)

caution Phenol:chloroform (1:1, v/v)

Restriction endonucleases

Please see Step 4.

recipe caution Sodium acetate (3 M, pH 5.2)

recipe TE (pH 8.0)


METHOD

1. Digest an amount . . . [Full Text of this Article]


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?