Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4413

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Rapid Preparation of DNA from Cells in 96-Well Tissue Culture Dishes

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Introduction of Foreign DNA into Embryonic Stem Cells," Chapter 10, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

It is important to minimize the time during which embryonic stem (ES) cells are in culture between selection and injection into blastocysts. This protocol describes a method for rapid preparation of DNA from cells in 96-well tissue culture dishes.


MATERIALS

Reagents

Bovine serum albumin (BSA)

ES cells in 96-well plate, confluent (see Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking)

Ethanol, 70% and prechilled 100%

Gel electrophoresis loading buffer

Gelatin, 0.1% solution (e.g., Sigma) in tissue culture-grade H2O

recipe Microtiter cell lysis . . . [Full Text of this Article]

Equipment


METHOD


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Related Protocol

Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4410. [Extract] [Full Text]