Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4411

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagy, A.
Right arrow Articles by Behringer, R.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Nagy, A.
Right arrow Articles by Behringer, R.
Related Collections
Right arrow CSHL Molecular Embryology of the Mouse Course
Right arrow Laboratory Organisms, general
Right arrow Mouse
Right arrow Developmental Biology
Right arrow Stem Cells, general
Right arrow Embryonic Stem Cells
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Freezing Embryonic Stem (ES) Cells in 96-Well Plates

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Introduction of Foreign DNA into Embryonic Stem Cells," Chapter 10, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for freezing ES cells in 96-well plates after ES colonies have been isolated by picking.


MATERIALS

Reagents

ES cells, in 96-well dish containing ES cells confluent in most of the wells (see Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking)

recipe ES cell culture medium with 15% fetal bovine . . . [Full Text of this Article]

Equipment


METHOD


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Protocol

Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4410. [Extract] [Full Text]