Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4406

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Culturing Trophoblast Stem (TS) Cell Lines

Janet Rossant

This protocol was adapted from "Isolation and Culture of Blastocyst-Derived Stem Cell Lines," Chapter 8 (Protocol 8, provided by the laboratory of Janet Rossant), in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

Recently, culture conditions have been established for a second blastocyst-derived cell line, trophoblast stem (TS) cells, in addition to embryonic stem (ES) cells. This protocol describes a method for culturing TS cell lines. These cells can then be used to study trophoblast differentiation and placental function.


MATERIALS

Reagents

caution Dimethyl sulfoxide (DMSO) (e.g., Sigma)

recipe Feeder-conditioned medium (feeder-CM)

recipe FGF4 stock solution (1000X, 25 µg/ml)

Heparin stock solution (1000X, 1.0 mg/ml) (Sigma, 10,000 units)

recipeResuspend in PBS and store at -80ºC. The stock can be also prepared as 10,000X (10 mg/ml) solution.

Mouse . . . [Full Text of this Article]

Equipment


METHOD


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