Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4388

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Blood Collection by Tail Bleeding

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Surgical Procedures," Chapter 6, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for collection of blood from the tail of a mouse. The blood can then be used for analysis.


MATERIALS

Reagents

recipe caution Appropriate anesthetic (depending on amount of blood to be collected, see Step 1.

cautionIf collecting up to 200 µl of blood use 0.5 ml Isofluorane.

recipe cautionIf collecting 200-500 µl of blood use Ketamine/Xylazine or Tribromoethanol (Avertin).

Heparin (optional)

Mouse

recipe Phosphate-buffered saline . . . [Full Text of this Article]

Equipment


METHOD


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