Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4387

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Castration

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Surgical Procedures," Chapter 6, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes castration of male mice, which is used to eliminate testicular hormones and/or to obtain testes for analysis without sacrificing the male. Castration is performed in a similar manner to vasectomy. Access to the testes can be achieved by either of two approaches (see Vasectomy for Generation of Sterile Males: Access via Abdominal Wall and Vasectomy for . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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