Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4385

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Splenectomy

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Surgical Procedures," Chapter 6, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes removal of a mouse spleen to provide tissue for analysis.


MATERIALS

Reagents

recipe caution Appropriate anesthetic

Ethanol, 70%

Mouse

Equipment

Animal clippers

Clean cage

Fiber-optic illuminator (optional)

Forceps, blunt

Forceps, watchmaker’s #5, two pairs

Needle, 26-gauge, 1/2 inch

Needle, curved surgical (e.g., size 10, . . . [Full Text of this Article]


METHOD


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Related Protocol

Intraperitoneal (IP) Injection
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4352. [Extract] [Full Text]