Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4382

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Transplantation of Tissues Under the Kidney Capsule

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Surgical Procedures," Chapter 6, (Protocol 6, information provided by Davor Solter) in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for transplanting tissue under the kidney capsule. The in vivo developmental potential of transplanted adult or embryonic tissues can then be determined.


MATERIALS

Reagents

Adult or embryonic tissue to be transplanted

recipe caution Appropriate anesthetic

Ethanol, 70%

Recipient histocompatible (or immunodeficient) mouse

recipe Sterile isotonic or phosphate-buffered saline (PBS) (pH 7.2)

Equipment

Clean cage

Drummond micropipette tip or sealing wax

Forceps, 26-mm diameter Desmarres chalazion (Shields/Dina C62-2810)

Forceps, blunt fine

Forceps, watchmaker’s #5

. . . [Full Text of this Article]


METHOD


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Related Protocol

Intraperitoneal (IP) Injection
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4352. [Extract] [Full Text]