Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4380

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Uterine Transfer

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Surgical Procedures," Chapter 6, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 100 words of the full text of this article appear below.


INTRODUCTION

This protocol describes a procedure for uterine transfer, which is used for chimera production. The method is based on extensive work which resulted in the first successful development and birth of in-vitro-cultured mouse embryos. It is best to practice this procedure first on a cadaver and then on an anesthetized 2.5-days post coitum (dpc) pseudopregnant mouse using blue Affigel beads rather than embryos.


MATERIALS

Reagents

recipe caution Appropriate anesthetic

Beads, blue Affigel (BioRad)

recipe Dulbecco’s modified Eagle’s medium (DMEM) with HEPES, M2 medium or any other HEPES-buffered medium

Ethanol, 70%

Mouse blastocysts (3.5 dpc) (see Collecting Blastocysts)

Pseudopregnant female recipient mice at 2.5 dpc . . . [Full Text of this Article]

Equipment


METHOD


TROUBLESHOOTING


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