Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4361

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Visualizing Early Embryo Implantation Sites by Dye Injection

Sudhansu K. Dey

This protocol was adapted from "Isolation, Culture, and Manipulation of Postimplantation Embryos," Chapter 5 (Protocol 1, procedure provided by S.K. Dey), in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for visualizing early embryo implantation sites using Chicago Sky Blue 6B dye. Once implantation and interimplantation sites are identified and separated, they can be used for cellular, biochemical, and molecular biology analyses.


MATERIALS

Reagents

recipe caution Appropriate anesthetic

recipe caution Chicago Sky Blue 6B

recipe caution

Chicago blue is also called Pontamine Sky Blue (Sigma)

Pregnant female mice (4.5-5.5 days . . . [Full Text of this Article]

Equipment


METHOD


TROUBLESHOOTING


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