Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4285

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Labeling Antibodies with Fluorochromes

Ed Harlow and David Lane

This protocol was adapted from "Handling Antibodies," Chapter 4, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 100 words of the full text of this article appear below.


INTRODUCTION

Direct labeling of purified antibodies is the method of choice when simultaneously visualizing two or more antibodies of the same species, class, or subclass. This allows the localization of multiple antigens to be compared in the same cell, tissue, or sample. Labeled primary antibodies are also useful for improving background-to-readout ratios, and they can be essential for immunoassays in which good quantification is needed. Fluorescent labels are necessary for cell sorting and high-resolution immunostaining, and they are also an excellent choice for determining precise subcellular localization. For other labeling methods, see Labeling Antibodies with Biotin, Labeling Antibodies with Iodine. . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


TROUBLESHOOTING


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