Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4281
| Protocol |
This protocol was adapted from "Handling Antibodies," Chapter 4, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.
| The first 15% of the full text of this article appears below. |
INTRODUCTION
Ascitic fluid from B-cell-derived tumors is an extremely concentrated solution of specific antibodies. Ascitic fluid is often stored at -70ºC to prevent proteolytic degradation
MATERIALS
Reagents
METHOD
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