Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4280

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Storage of Tissue Culture Supernatants

Ed Harlow and David Lane

This protocol was adapted from "Handling Antibodies," Chapter 4, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes the methods of storage for hybridoma tissue-culture supernatants. Antibodies are resistant to a broad range of mildly denaturing conditions, . . . [Full Text of this Article]


MATERIALS

Reagents


METHOD


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