Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4391

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Purification of Bacterial Artificial Chromosome (BAC) DNA Prepared with the NucleoBond System

Thom Saunders

This protocol was adapted from "Production of Transgenic Mice" in Chapter 7 of Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes purification of BAC DNA that has been prepared with the commercial NucleoBond system. . . . [Full Text of this Article]


MATERIALS

Reagents


METHOD


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Related Protocol

Isolating Bacterial Artificial Chromosome (BAC) DNA from Bacterial Cultures Using the NucleoBond System
Thom Saunders
CSH Protocols 2006: 4390. [Extract] [Full Text]