Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4390

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Isolating Bacterial Artificial Chromosome (BAC) DNA from Bacterial Cultures Using the NucleoBond System

Thom Saunders

This protocol was adapted from "Production of Transgenic Mice," Chapter 7 of Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes isolation of BAC DNA from bacterial cultures using the commercial NucleoBond system.


MATERIALS

Reagents

Bacterial culture pellet, from appropriate 100- to 300-ml bacterial culture prepared according to standard methods

NucleoBond Buffer Set I (Clontech)

Store Buffers S1 and S3 at 4°C; Buffers S2, N2, N3, and N5 at room temperature.

Equipment

NucleoBond AX-500 Tip (Clontech)

NucleoBond Folded Filters (Clontech)

Tube, . . . [Full Text of this Article]


METHOD


TROUBLESHOOTING


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