Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4378

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Ovariectomy for Induction of Blastocyst Implantation Delay

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Surgical Procedures," Chapter 6 of Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for removal of mouse ovaries in combination with the administration of progesterone, which causes the arrest of blastocyst development. This procedure is performed during the afternoon of the third day of pregnancy to ensure that the morulae have moved sufficiently into the oviducts and will be less likely to be damaged during the ovariectomy.


MATERIALS

Reagents

recipe caution Tribromoethanol (Avertin), or other appropriate anesthetic,

Depo-Provera (6{alpha}-methyl-17{alpha}-hydroxy-progesterone acetate) solution (10 mg/ml in sterile . . . [Full Text of this Article]

Equipment


METHOD


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