Protocol

Electrophoresis of Peptides (Tricine-SDS-PAGE)

This protocol was adapted from "Concentrating Solutions of Protein," in Appendix 5 of Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

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INTRODUCTION

Typical Laemmli gel systems, which use glycine in the running buffer, are capable of resolving proteins in the molecular mass range of ~200,000 Da down to ~3000 Da. In this protocol, the standard tricine gel system is described, in which tricine . . . [Full Text of this Article]

MATERIALS

Reagents

Equipment

METHOD