Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4312

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Precipitation of Proteins by Polyethylenimine

Richard J. Simpson

This protocol was adapted from "Concentrating Solutions of Protein," in Appendix 3 of Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

The first 100 words of the full text of this article appear below.


INTRODUCTION

Polyethylenimine (PEI) is an aliphatic polyamine polymer available in a broad range of molecular weights in both linear and multiply branched forms. Because of its high molecular weight and very high cationic charge, PEI is a powerful modifier of surface charge on proteins. The binding of proteins to PEI is salt-dependent. Precipitated proteins are recovered from PEI-protein complexes by elution with NaCl. Ammonium sulfate precipitation is often used after PEI precipitation and elution, because protein will be precipitated by ammonium sulfate, but the PEI will remain in solution.


MATERIALS

Reagents

caution Ammonium sulfate (analar grade)

recipe NaCl buffer

recipe caution PEI reagent: 10% (w/v) PEI in . . . [Full Text of this Article]

Equipment


METHOD


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