Protocol

Precipitation of Proteins by Polyethylene Glycol

This protocol was adapted from "Concentrating Solutions of Protein," in Appendix 3 of Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

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INTRODUCTION

When used at appropriate concentrations, polyethylene glycol (PEG), which is nonionic and water-soluble, can cause proteins to precipitate from solution. Proteins of higher mass tend to precipitate from solution at lower concentrations of PEG than do smaller proteins. Maximum protein precipitation of bulk protein is usually achieved with a final PEG concentration of 30% and is relatively insensitive to ionic strength and pH. A major advantage of . . . [Full Text of this Article]

MATERIALS

Reagents

Equipment

METHOD