Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4307

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Protein Quantitation: UV Detection

Ed Harlow and David Lane

This protocol was adapted from "Protein Techniques," Appendix II, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

Absorbance of UV irradiation by proteins is the quickest of all methods for quantitating protein solutions, and none of the sample is destroyed in the process. Readings are most . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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