Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4306

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Protein Quantitation: Bradford Spot Test

Ed Harlow and David Lane

This protocol was adapted from "Protein Techniques," Appendix II, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

This assay is particularly useful for testing affinity column fractions to locate the protein eluate.


MATERIALS

Reagents

recipe Bradford dye concentrate

Equipment

Microwell tray, . . . [Full Text of this Article]


METHOD


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